Depolarization-Stimulated K Efflux in Rat Aorta is Calcium- and Cellular Volume-Dependent

The purpose of this study was to investigate the factors controlling membrane permeability to potassium of smooth muscle cells from rat aorta stimulated by depolarization. The increase in K + efflux (change in the rate constant) induced by depolarization (application of high concentrations of potassium chloride) was inhibited significantly by the calcium antagonists diltiazem and nisoldipine. Parallel inhibitory effects on contraction were observed. Diltiazem also inhibited potassium-stimulated CI ~ efflux. The addition of 25-150 mM KCI to normal physiologic solution stimulated K + efflux in a concentration-dependent manner. Diltiazem suppressed potassium-stimulated K efflux approximately 90% at 25 mM KCI and approximately 40% at 150 mM KCI. The ability of nisoldipine to inhibit K + efflux also diminished as the potassium chloride concentration was elevated. The component of efflux that was resistant to calcium antagonists probably resulted from a decrease in the electrochemical gradient for potassium. Cellular water did not change during potassium addition. Substitution of 80 and 150 mM KCI for sodium chloride produced cellular swelling and enhanced potassium-stimulated K + efflux compared with potassium chloride addition. The addition of sucrose to prevent cellular swelling reduced efflux response to potassium substitution toward that of potassium addition. A hvpoosmolar physiologic solution produced an increase in theK efflux and a contracture that were both prevented by the addition of sucrose. We concluded that the depolarization-mediated K + efflux has three components: one is calcium dependent; a second is dependent on cellular volume; and a third is resistant to inhibition by calcium antagonists. (Circulation Research